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pbluescript ii ks vector  (New England Biolabs)


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    Structured Review

    New England Biolabs pbluescript ii ks vector
    Pbluescript Ii Ks Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbluescript+ii+ks+vector/pm38087090-257-6-13
    Average 86 stars, based on 1 article reviews
    pbluescript ii ks vector - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Clone Assay:

    Article Title: The anticancer compound JTE-607 reveals hidden sequence specificity of the mRNA 3' processing machinery.
    Article Snippet: DNA oligos containing 23 random nucleotide CS was purchased from IDT and PCR amplified to generate double-stranded (ds)DNA. .. The dsDNA library was cloned into pBlueScript II KS+ vector by Gibson Assembly (NEB) and electroporated into ElectroMAX DH5α (Thermo Fisher Scientific). ..

    Article Title: Genetic Dissection of BDNF and TrkB Expression in Glial Cells
    Article Snippet: .. The DNA donor plasmid contains two homology arms with 840 bp flanking the P2A-Cre sequence, which were cloned into BamHI-digested pBluescript II KS (-) vector using the Gibson assembly method (NEB, Ipswich, MA, USA, E5510). .. To block further Cas9 targeting and recutting after undergoing homology-direct repair, we introduced a silent mutation into the PAM motif of the sgRNA located within the 3′ homology arm in the donor plasmid by using Q5 Site-Directed Mutagenesis kit (NEB, E0054).

    Article Title: Protein kinase C activity modulates nuclear Lamin A/C dynamics in HeLa cells
    Article Snippet: .. This region was then cloned into the LMNA translation start site in the pBlueScript II KS (−) vector after digestion with NcoI-HF (New England BioLabs, R3193S). ..

    Article Title: Comparing the Conformational Stability of Pyruvate Kinase in the Gas Phase and in Solution.
    Article Snippet: Collision induced unfolding (CIU) is increasingly used to characterize protein complexes in the gas phase and is often employed to detect ligand binding-induced conformational stabilization.. However, the extent to which gas-phase conformational stabilities measured by CIU reflect analogous parameters in solution is not yet clear, particularly for systems where conformational and protein complex stability are modulated by point mutation.. Here, we compare CIU-derived relative stabilities of four point mutants of the homotetramer pyruvate kinase to solution stabilities measured by differential scanning fluorimetry (DSF) and solution conformational dynamics measured by time-resolved electrospray ionization hydrogen−deuterium exchange (TRESI-HDX).

    Article Title: Genetic Dissection of BDNF and TrkB Expression in Glial Cells.
    Article Snippet: .. The DNA donor plasmid contains two homology arms with 840 bp flanking the Biomolecules 2024, 14, 91 3 of 18 P2A-Cre sequence, which were cloned into BamHI-digested pBluescript II KS (-) vector using the Gibson assembly method (NEB, Ipswich, MA, USA, E5510). .. To block further Cas9 targeting and recutting after undergoing homology-direct repair, we introduced a silent mutation into the PAM motif of the sgRNA located within the 3′ homology arm in the donor plasmid by using Q5 Site-Directed Mutagenesis kit (NEB, E0054).

    Plasmid Preparation:

    Article Title: The anticancer compound JTE-607 reveals hidden sequence specificity of the mRNA 3' processing machinery.
    Article Snippet: DNA oligos containing 23 random nucleotide CS was purchased from IDT and PCR amplified to generate double-stranded (ds)DNA. .. The dsDNA library was cloned into pBlueScript II KS+ vector by Gibson Assembly (NEB) and electroporated into ElectroMAX DH5α (Thermo Fisher Scientific). ..

    Article Title: Genetic Dissection of BDNF and TrkB Expression in Glial Cells
    Article Snippet: .. The DNA donor plasmid contains two homology arms with 840 bp flanking the P2A-Cre sequence, which were cloned into BamHI-digested pBluescript II KS (-) vector using the Gibson assembly method (NEB, Ipswich, MA, USA, E5510). .. To block further Cas9 targeting and recutting after undergoing homology-direct repair, we introduced a silent mutation into the PAM motif of the sgRNA located within the 3′ homology arm in the donor plasmid by using Q5 Site-Directed Mutagenesis kit (NEB, E0054).

    Article Title: Genetic Dissection of BDNF and TrkB Expression in Glial Cells.
    Article Snippet: .. The DNA donor plasmid contains two homology arms with 840 bp flanking the Biomolecules 2024, 14, 91 3 of 18 P2A-Cre sequence, which were cloned into BamHI-digested pBluescript II KS (-) vector using the Gibson assembly method (NEB, Ipswich, MA, USA, E5510). .. To block further Cas9 targeting and recutting after undergoing homology-direct repair, we introduced a silent mutation into the PAM motif of the sgRNA located within the 3′ homology arm in the donor plasmid by using Q5 Site-Directed Mutagenesis kit (NEB, E0054).

    Sequencing:

    Article Title: Genetic Dissection of BDNF and TrkB Expression in Glial Cells
    Article Snippet: .. The DNA donor plasmid contains two homology arms with 840 bp flanking the P2A-Cre sequence, which were cloned into BamHI-digested pBluescript II KS (-) vector using the Gibson assembly method (NEB, Ipswich, MA, USA, E5510). .. To block further Cas9 targeting and recutting after undergoing homology-direct repair, we introduced a silent mutation into the PAM motif of the sgRNA located within the 3′ homology arm in the donor plasmid by using Q5 Site-Directed Mutagenesis kit (NEB, E0054).

    Article Title: Genetic Dissection of BDNF and TrkB Expression in Glial Cells.
    Article Snippet: .. The DNA donor plasmid contains two homology arms with 840 bp flanking the Biomolecules 2024, 14, 91 3 of 18 P2A-Cre sequence, which were cloned into BamHI-digested pBluescript II KS (-) vector using the Gibson assembly method (NEB, Ipswich, MA, USA, E5510). .. To block further Cas9 targeting and recutting after undergoing homology-direct repair, we introduced a silent mutation into the PAM motif of the sgRNA located within the 3′ homology arm in the donor plasmid by using Q5 Site-Directed Mutagenesis kit (NEB, E0054).

    Purification:

    Article Title: Comparing the Conformational Stability of Pyruvate Kinase in the Gas Phase and in Solution.
    Article Snippet: Collision induced unfolding (CIU) is increasingly used to characterize protein complexes in the gas phase and is often employed to detect ligand binding-induced conformational stabilization.. However, the extent to which gas-phase conformational stabilities measured by CIU reflect analogous parameters in solution is not yet clear, particularly for systems where conformational and protein complex stability are modulated by point mutation.. Here, we compare CIU-derived relative stabilities of four point mutants of the homotetramer pyruvate kinase to solution stabilities measured by differential scanning fluorimetry (DSF) and solution conformational dynamics measured by time-resolved electrospray ionization hydrogen−deuterium exchange (TRESI-HDX).

    Gel Extraction:

    Article Title: Comparing the Conformational Stability of Pyruvate Kinase in the Gas Phase and in Solution.
    Article Snippet: Collision induced unfolding (CIU) is increasingly used to characterize protein complexes in the gas phase and is often employed to detect ligand binding-induced conformational stabilization.. However, the extent to which gas-phase conformational stabilities measured by CIU reflect analogous parameters in solution is not yet clear, particularly for systems where conformational and protein complex stability are modulated by point mutation.. Here, we compare CIU-derived relative stabilities of four point mutants of the homotetramer pyruvate kinase to solution stabilities measured by differential scanning fluorimetry (DSF) and solution conformational dynamics measured by time-resolved electrospray ionization hydrogen−deuterium exchange (TRESI-HDX).



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